Skip to main content

Log in
|
Help
|
English

English

Deutsch

Espanol

Français

Search

Advanced Search

Find results with:

Add another field

Search by date

Search by date:
from:
to
to:

Searching collections:

Augustana Student Research

Add or remove collections

Home

Augustana Student Research

Characterization of antibacterial products produced by a bacterial symbiont of the burying beetle...

Reference URL

To link to this object, paste this link in email, IM or document
To embed this object, paste this HTML in website

Characterization of antibacterial products produced by a bacterial symbiont of the burying beetle Nicrophorus marginatus

View Description

This item is restricted to only allow viewing of the metadata.

you wish to report:

Your comment:

Your Name:

...

Back to top

Powered by CONTENTdm®

Title	Characterization of antibacterial products produced by a bacterial symbiont of the burying beetle Nicrophorus marginatus
Author	Anderson, Matthew P.
Co-authors	Moore,Courtney L.
Subject (LC)	Burying beetles Host-bacteria relationships
Abstract	The burying beetle Nicrophorous marginatus is known to produce oral and anal secretions that have antibacterial properties. In this study, bacteria were isolated from beetle secretions and screened for production of antibacterial compounds that could contribute to the antibacterial activity. One isolate, designated Bacillus-17, was found to produce an antibacterial factor that was effective against 18 of 23 bacterial species tested, including Staphylococcus aureus, Staphylococcus epidermidis and a variety of soil bacteria. The 16S rRNA gene sequence obtained from this isolate indicates the bacterium is a member of the genus Bacillus. Cell-free culture supernatants of Bacillus-17 were able to inhibit growth of Staphylococcus aureus. This activity was inhibited by Proteinase K treatment. Fractionation of cell-free culture supernatants showed that the antibacterial factor had a molecular weight of >9 kDa. The antibacterial factor was capable of lysing whole S. aureus cells and hydrolyzing peptidoglycan, the major component of bacterial cell walls. SDS-PAGE was used to separate the active fraction, and 8 proteins were observed. These results suggest the antibacterial factor produced by Bacillus-17 may be a lysozyme that has yet to be characterized.
Research affiliation	BRIN (Biomedical Research Infrastructure Network)
Collection	Augustana Student Research
Date	2012
Rights	This image may not be reproduced without the express written consent of Augustana University, Sioux Falls, South Dakota, USA.
Type	Poster
Medium	Image
Format - Digital	pdf