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Characterization of antibacterial products produced by a bacterial symbiont of the burying beetle...

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Characterization of antibacterial products produced by a bacterial symbiont of the burying beetle Nicrophorus marginatus

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Title	Characterization of antibacterial products produced by a bacterial symbiont of the burying beetle Nicrophorus marginatus
Author(s)	Anderson, Matthew P.
Co-author(s)	Moore,Courtney L.
Abstract	The burying beetle Nicrophorous marginatus is known to produce oral and anal secretions that have antibacterial properties. In this study, bacteria were isolated from beetle secretions and screened for production of antibacterial compounds that could contribute to the antibacterial activity. One isolate, designated Bacillus-17, was found to produce an antibacterial factor that was effective against 18 of 23 bacterial species tested, including Staphylococcus aureus, Staphylococcus epidermidis and a variety of soil bacteria. The 16S rRNA gene sequence obtained from this isolate indicates the bacterium is a member of the genus Bacillus. Cell-free culture supernatants of Bacillus-17 were able to inhibit growth of Staphylococcus aureus. This activity was inhibited by Proteinase K treatment. Fractionation of cell-free culture supernatants showed that the antibacterial factor had a molecular weight of >9 kDa. The antibacterial factor was capable of lysing whole S. aureus cells and hydrolyzing peptidoglycan, the major component of bacterial cell walls. SDS-PAGE was used to separate the active fraction, and 8 proteins were observed. These results suggest the antibacterial factor produced by Bacillus-17 may be a lysozyme that has yet to be characterized.
Subject (LC)	Burying beetles Host-bacteria relationships
Research affiliation	BRIN (Biomedical Research Infrastructure Network)
Collection	Augustana Student Research
Date	2012
Rights	This image may not be reproduced without the express written consent of Augustana University, Sioux Falls, South Dakota, USA.
Type	Poster
Medium	Image
Format - Digital	pdf